{"created":"2023-06-20T15:05:28.318839+00:00","id":2101,"links":{},"metadata":{"_buckets":{"deposit":"51b4842b-22e7-4594-a8f5-dc6eef4d7f7e"},"_deposit":{"created_by":3,"id":"2101","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2101"},"status":"published"},"_oai":{"id":"oai:osaka-shoin.repo.nii.ac.jp:00002101","sets":["160:167"]},"author_link":["2669","9542","2674","9543","2938","9544"],"control_number":"2101","item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"コウコウ ネンマク サイボウ カラノ total RNA チュウシュツホウ ノ ケントウ","subitem_alternative_title_language":"ja"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"136","bibliographicPageStart":"129","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"大阪樟蔭女子大学学芸学部論集"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"籾谷, 真奈","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木村, 雅浩","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"モミタニ, マナ","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"キムラ, マサヒロ","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{}]},{"creatorNames":[{}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"口腔粘膜細胞からtotal RNAを抽出するための条件検討を行った。一般的な、グアニジンチオシアネートを用いた方法よりも、SDSを用いた方法のほうがtotal RNAの抽出に適しており、この際、proteinase Kの添加が必要であることを明らかにした。また競合的RT-PCRの結果、ブラシで口腔粘膜細胞を剥離して採取した方が、口腔洗浄液から採取した口腔粘膜細胞よりもβ-actinを増幅するのに適していることがわかった。これらの結果から、口腔粘膜細胞からtotal RNAを抽出するには、ブラシによる口腔粘膜細胞の採取とSDSによる抽出が適していることがわかった。","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11643143","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Osaka Shoin Women's University/Osaka Shoin Women's University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"大阪樟蔭女子大学学芸学部/大阪樟蔭女子大学学芸学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2008-01-31"}],"displaytype":"detail","filename":"KJ00004877220.pdf","filesize":[{"value":"919.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"KJ00004877220","url":"https://osaka-shoin.repo.nii.ac.jp/record/2101/files/KJ00004877220.pdf"},"version_id":"55a812fb-94b5-494b-bb18-69e3d03edb3f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"口腔粘膜細胞からのtotal RNA抽出法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"口腔粘膜細胞からのtotal RNA抽出法の検討","subitem_title_language":"ja"},{"subitem_title":"Isolation of total RNA from buccal mucosal cells","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["167"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-01-31"},"publish_date":"2008-01-31","publish_status":"0","recid":"2101","relation_version_is_last":true,"title":["口腔粘膜細胞からのtotal RNA抽出法の検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-12-06T05:00:00.490090+00:00"}