{"created":"2023-06-20T15:05:27.300009+00:00","id":2057,"links":{},"metadata":{"_buckets":{"deposit":"ea3143a6-7970-4e58-9955-93da7b393831"},"_deposit":{"created_by":3,"id":"2057","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2057"},"status":"published"},"_oai":{"id":"oai:osaka-shoin.repo.nii.ac.jp:00002057","sets":["160:166"]},"author_link":["2771","2772","2773"],"control_number":"2057","item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"Bacillus circulans G22-10 ユライ カンジョウ イソマルト オリゴトウ ゴウセイ コウソ CITase イデンシ ノ クローニング ト ダイチョウキン ハツゲンケイ ノ サクセイ","subitem_alternative_title_language":"ja"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"67","bibliographicPageStart":"61","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"大阪樟蔭女子大学学芸学部論集"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川端康之","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"カワバタ, ヤスユキ","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"Bacillus circulans G22-10由来環状イソマルトオリゴ糖合成酵素(CITase)の大量調製を目的として、同酵素遺伝子のクローニングと大腸菌を宿主とする高発現系の構築を試みた。 B. circulans G22-10のゲノムからPCR法により増幅したCITase遺伝子を、pET-15bのNdeI-BamHIサイトに組込み、同遺伝子クローニングベクターpCITを構築した。pCITを用いて発現用宿主E.coliBL21(DE3)pLysSを形質変換し、CITaseを生産するBL21(pCIT)を得た。BL21(pCIT)をインスタントTB培地で培養することで、著量のCITaseが菌体内に可溶性タンパク質として発現された。菌体を破砕し粗酵素液を調製後、Ni-NTA アガロースカラムクロマトグラフィーで精製した。精製酵素はSDS-PAGEでシングルバンドとして検出され、ザイモグラフィーの酵素活性と一致した。大腸菌組換え型CITaseとG22-10由来の非組換え型CITaseの諸性質を検討したところ、至適温度、至適ph、温度安定性、ph安定性は同様であった。また、デキストランを基質としたときの生産物特異性についても、同様であった。大腸菌組換え型にはN-末端に21アミノ酸の付加配列が存在するが、酵素の性質には全く影響がないと結論した。","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11643143","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Osaka Shoin Women's University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"大阪樟蔭女子大学学芸学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-03-20"}],"displaytype":"detail","filename":"KJ00004571260.pdf","filesize":[{"value":"988.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"url":"https://osaka-shoin.repo.nii.ac.jp/record/2057/files/KJ00004571260.pdf"},"version_id":"3787b5f1-8357-4155-a110-7284c82143c3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bacillus circulans G22-10由来環状イソマルトオリゴ糖合成酵素(CITase)遺伝子のクローニングと大腸菌発現系の作成","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacillus circulans G22-10由来環状イソマルトオリゴ糖合成酵素(CITase)遺伝子のクローニングと大腸菌発現系の作成","subitem_title_language":"ja"},{"subitem_title":"Molecular Cloning of a Cycloisomatooligosaccharide Glucanotransferase (CITase) from Bacillus circulans G22-10 and Its Expression in Eschelichia coli","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["166"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-03-20"},"publish_date":"2007-03-20","publish_status":"0","recid":"2057","relation_version_is_last":true,"title":["Bacillus circulans G22-10由来環状イソマルトオリゴ糖合成酵素(CITase)遺伝子のクローニングと大腸菌発現系の作成"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-12-14T07:48:55.334855+00:00"}