{"created":"2023-06-20T15:05:24.770421+00:00","id":1893,"links":{},"metadata":{"_buckets":{"deposit":"f90995c3-4d5a-4a8f-9975-f8996b4cb50d"},"_deposit":{"created_by":3,"id":"1893","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1893"},"status":"published"},"_oai":{"id":"oai:osaka-shoin.repo.nii.ac.jp:00001893","sets":["160:163"]},"author_link":["2014","2015","2016"],"control_number":"1893","item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"ダイチョウキン デ ハツゲンサセタ P450 CYP2D6 オ コソウ コウゲン ト シタ ELISAホウ ノ カイハツ","subitem_alternative_title_language":"ja"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"98","bibliographicPageStart":"91","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"大阪樟蔭女子大学学芸学部論集"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木村, 雅浩","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"キムラ, マサヒロ","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"自己免疫性肝炎は、病状の進行が早く、早期に適切な治療が行われないと予後が悪い。したがって、早期診断、早期治療が重要である。AIHの確定診断の有力な候補として、「抗CYP2D6抗体」の測定が試みられている。「抗CYP2D6抗体」は、AIH患者血清中に検出される特異性の高い自己抗体であるが、現在の測定方法では測定感度が低く、確定診断には到っていない。そこで、より汎用性があり、多くの自己抗体の測定法としての実用例のあるELISA法での抗体測定を試みた。 大腸菌で発現させたP450 CYP2D6Δ25を固相抗原として抗CYP2D6抗体測定用ELISAプレートを作成した。P450 CYP2D6Δ25の発現の最適条件は、培養液のOD600nmでの吸光度が0.6～0.8の間に誘導を開始し、誘導3時間後に、菌体を回収するのが良いことが分かった。また、粗精製したP450 CYP2D6Δ25を固相抗原としてELISAを行ったところ、抗体濃度の希釈にしたがい直線的な吸光度の減少が認められ、定量的に抗CYP2D6抗体を測定できることが分かった。今後は、さらに、P450 CYP2D6Δ25の精製度をあげ、患者血清中の抗CYP2D6抗体測定する必要がある。","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11643143","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Osaka Shoin Women's University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"大阪樟蔭女子大学学芸学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2004-03-06"}],"displaytype":"detail","filename":"KJ00000236254.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"url":"https://osaka-shoin.repo.nii.ac.jp/record/1893/files/KJ00000236254.pdf"},"version_id":"cf0b3df6-2a7f-4829-868d-83a310b882ca"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"大腸菌で発現させたP450 CYP2D6を固相抗原としたELISA法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大腸菌で発現させたP450 CYP2D6を固相抗原としたELISA法の開発","subitem_title_language":"ja"},{"subitem_title":"Development of the Enzyme-linked Immunosorbent Assay (ELISA) for Anti-P450 CYP2D6 Antibodies","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["163"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2004-03-06"},"publish_date":"2004-03-06","publish_status":"0","recid":"1893","relation_version_is_last":true,"title":["大腸菌で発現させたP450 CYP2D6を固相抗原としたELISA法の開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-12-20T07:21:27.562915+00:00"}